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Electrophoresis and western blotting Proteins were extracted from the frozen tissue or cells harvested via trypsinization by homogenisation in a 2% SDS and 62.5 mM Tris and were heat-denatured at 90 C for 10 min, then centrifuged at 21,000 g for 30 min at 4 C

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Additionally, the Nrg4/ErbB4/AKT signaling axis promotes the phosphorylation of cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS), reducing its enzymatic activity and thereby inhibiting hepatic inflammation and steatosis mediated by the cGAS-STING pathway [108]

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Altogether, these results demonstrate that glutamine functions as a TCA cycle carbon donor and a nitrogen donor in the subsequent production of aspartate in sarcoma